Chimeric phage
WebSep 5, 2008 · Since GFP and chimeric protein expressed in cells are less fluorescent at high culture temperature , visualization of E. coli cells by GFP-labeled phage infection was conducted at 28 °C. Fluorescent microscopic images of E. coli B E cells after infection with IP008e-/2xGFP or IP052e-/2xGFP indicated that the fluorescent intensity of E. coli B ... WebChimeric Vaccine. For example, chimeric vaccines have been developed in which the genes encoding the envelope proteins of the traditional live-attenuated vaccine strain of …
Chimeric phage
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WebJan 1, 2012 · The first phage expresses the 1B6 peptide on recombinant protein VIII and is a type 88 chimeric phage. The second phage type displays the 1B6 peptide on all copies of protein III. It should be noted that only insert expression on recombinant protein VIII generates the GIL sequence. WebDec 1, 2024 · With the increasing spread of multidrug-resistant bacterial pathogens, it is of great importance to develop alternatives to conventional antibiotics. Here, we report the generation of a chimeric phage lysin, …
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WebOct 16, 2024 · Phage-encoded lysins (i.e., endolysins) are cell wall hydrolases that work in concert with holins to release progeny phage during a lytic infection cycle [chapter … WebSep 13, 2005 · All three chimeric phages are viable ( Figure 4 ). We isolated DNA and performed restriction digests across alpha and beta to confirm that individual parts could be independently manipulated. We …
WebMay 5, 2024 · We recently reported a phage-based strategy for rapid, sensitive, and specific detection of bacteria using engineered M13, in which the receptor-binding protein (RBP) …
WebNov 28, 2024 · This study has constructed a new chimeric lysin, Csl2, by fusing the catalytic domain of Cpl-7 lysozyme to the CW_7 repeats of LySMP lys in from an S. suis phage, and shows noticeable activity against a few streptococci of the mitis group. Streptococcus suis is a Gram-positive bacterium that infects humans and various … orchestrator salaryWebMar 24, 2024 · These results exemplify the use of phage display biopanning for the isolation of GSC-targeting peptides, and their potential application in the development of novel cytotoxic therapies for GBM. ... To develop cytotoxic cellular products aimed at specifically targeting GSCs, chimeric antigen receptors (CARs) were engineered containing the ... ipwireless portalWebOct 29, 2024 · phage engineering tail spike receptor binding protein X-ray crystallography host range directed evolution synthetic biology chimeric phage Introduction Modulation of microbiomes through targeted … orchestrator runbook service not startingWebOct 1, 1990 · Chimeric genes were constructed by site-directed mutagenesis: a unique SnaBI restriction site in the cpl1 gene, coding for the phage lysozyme, was introduced at … ipwireless ia246-2500tWebJan 15, 2024 · As the incidence of antibiotic-resistant bacteria has become increased, phage endolysins are believed as one of the promising alternatives to antibiotics. However, the discovery of potent endolysin is still challenging because it is labor intensive and difficult to obtain a soluble form with high lytic activity. In this respect, the modular structures of … ipwireless incWebJan 25, 2024 · The results revealed that the wild-type phage PE3 and the chimeric phage PE3Δgp6–gp12 have a similar growth curve, while phages PE3Δgp1–gp5 and PE3Δgp1–gp12 have 5 min and 15 min longer ... orchestrator salesforceWebJan 2, 2024 · After the screening, selected leads are used to generate chimeric or humanized antibodies. b Phage display. A human phage-displayed human antibody library is used to select antigens of interest. After 3–5 rounds of biopanning, immuno-positive phage clones are screened by ELISA; then DNA sequences are analyzed to construct … orchestrator sandbox