Ectopic expression assay

Author: vmon

August undefined, 2024

WebWestern blot analysis revealed that ectopic expression of miR-26a, -145, -199a, -542-3p, and -543 significantly reduced the level of COX-2 protein. The inhibitory effect of miR-143, -542-3p, and -543 on COX-2 expression appeared to be mediated via interaction with predicted binding sites in the 3′-untranslated region (3′-UTR) of COX-2 as ... WebMoreover, the studies involved a variety of techniques and analysis methods to report the expression profiles of control and ectopic groups, making the data heterogenous. Indeed, future studies will evolve our database, but with the current knowledge we have provided the most comprehensive resource that will aid in the future research in this area.

The dysregulation of leukemia inhibitory factor and its implications ...

WebThe occurrence of gene expression in a tissue in which it is normally not expressed. Such ectopic expression can be caused by the juxtaposition of novel enhancer elements to a … WebApr 24, 2024 · Ectopic Expression Analysis of FaesAP1 in Arabidopsis ap1-10 Mutant. Full-length FaesAP1 cDNA in the sense orientation were cloned into pBI121 vector (BD Biosciences, Clontech) with Xba I and Sac I restriction enzymes under control of the CaMV35S promoter. atahobia

Ectopic expression of peripheral-tissue antigens in the …

WebEctopic pregnancy (EP) is one of the leading causes of maternal mortality, where the fertilized embryo grows outside of the uterus. Recent experiments on mice have … WebMoreover, the studies involved a variety of techniques and analysis methods to report the expression profiles of control and ectopic groups, making the data heterogenous. … WebJun 26, 2015 · LGALS1/galectin-1 and LGALS3/galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and … asian pfp gif

EPEK: Creation and analysis of an Ectopic Pregnancy …

Ectopic expression of the Dlx genes induces glutamic acid …

WebSep 6, 2024 · The B-genome–derived VRT-B2 gene in 3962 shows a normal expression pattern identical to that in JY8 (Figure 2 A), indicating that the ectopic expression of VRT2 is most likely A-genome specific. Further in situ hybridization assays showed that VRT-A2 was predominantly expressed in glumes and spikelet meristems in 3962 spikes ( Figure … WebJun 8, 2024 · The ectopic expression of MaAN2 in Nicotiana tabacum resulted in obvious red coloration of the leaves and much redder flowers. Almost all anthocyanin biosynthetic genes were remarkably upregulated in the leaves and flowers of the transgenic tobacco, and NtAn1a and NtAn1b (two basic helix-loop-helix anthocyanin regulatory genes) were … atahliah ayliffeWebJul 21, 2006 · In conclusion, ectopic expression of KNOX proteins induces specific IPT gene expression and de novo CK biosynthesis, and this cascade is conserved in both monocots and dicots. It is noteworthy that another important function of KNOX proteins—repression of GA biosynthesis through suppression of GA 20-oxidase gene … atahockey

"WebDec 13, 2024 · Heat-shock protein 90 (Hsp90) is a molecular chaperone protein that helps other proteins (referred to as ‘clients’) fold and remain stable in their active conformation 1,2,3.Many functions of ... " - Ectopic expression assay

Ectopic expression assay

WebOct 27, 2024 · To investigate whether Sim1 Het mice show ectopic expression of Irx3 and Irx5, we performed RNA in situ hybridization and quantitative ... (Thermo Fisher Scientific) with oligo(dT). Gene expression assay was conducted using SYBR Green methods on Viia7 (Applied Biosystems), and relative cycle threshold values were normalized by Actb … WebSep 26, 2013 · Reporter gene expression levels and flow cytometry assay results also supported the idea that cell division occurred in sef hypocotyl due to WUS ectopic expression (Figures 2, 3). Ectopic expression of WUS driven by the 35S promoter occasionally causes activation of the CycB1;1::GUS reporter gene along the vasculature …

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WebMar 23, 2024 · 1. Introduction. Endometriosis, a chronic inflammatory gynaecological disease, is defined by the growth of endometrial like tissue outside of the uterus ().Lesions, referred to as ectopic tissue, can manifest throughout the abdominal cavity constituting the subtypes of endometriosis based on lesion placement and depth: superficial peritoneal, …

WebSep 29, 2015 · Ectopic expression of WRI1 has also been used to stimulate oil production in nonseed tissues in both Arabidopsis and tobacco (Nicotiana tabacum; Cernac and Benning, ... Phylogenetic analysis by MEGA6 (Tamura et al., 2013) showed that the protein encoded by Bradi4g43877 was the closest ortholog to AtWRI1 . WebMar 1, 2012 · The terms “misexpression” and “ectopic expression” predominate in studies using metazoans, implying expression of a gene in a cell type or developmental stage or condition in which it normally is not expressed. Because the underlying genetic principles …

WebY. Doyon, J. Côté, in Methods in Enzymology, 2016 2.10 Dox-Inducible Expression of EZH2 from the AAVS1 Safe Harbor Locus. Ectopic expression of some chromatin … WebMar 26, 2024 · Expression profiles of VvBAM genes under different growth periods in the phloem of grapevine. The results of quantitative real-time polymerase chain reaction (qRT-PCR) showed that the six VvBAM genes had different expression patterns during the LT dormancy. The relative expression of VvBAM1 increased as temperature decreased, …

WebAug 21, 2024 · Tau is a microtubule (MT)-associated protein that is localized to the axon. In Alzheimer's disease, the distribution of tau undergoes a remarkable alteration, leading to the formation of tau inclusions in the somatodendritic compartment. To investigate how this mislocalization occurs, we recently developed immunohistochemical tools that can …

WebMar 16, 2024 · Key message Ectopic expression of Glycine max two-component system member GmHP08 in Arabidopsis enhanced drought tolerance of transgenic plants, … asian peterWebSep 1, 2015 · Transgenic assays, despite being relatively low throughput, represent an entirely orthogonal approach to those described above and, therefore, provide … atahortaWebDec 13, 2024 · Leveraging ectopic Hsp90 expression to assay the presence of tumor cells and aggressive tumor phenotypes in breast specimens Download PDF Your article has downloaded atahli okcWebJan 6, 2015 · The transient expression assay using Arabidopsis protoplasts showed that FtMYB12 fused to the GFP was localized in the nucleus ... He Y, Li W, Lv J, Jia Y, Wang M, Xia G (2012) Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana. J Exp Bot 63:1511–1522 asian petsWebOct 27, 2024 · Ectopic expression of Irx3 and Irx5 in the Sim1-Cre + PVH cells of Sim1 Het mice. (A) ... (Thermo Fisher Scientific) with oligo(dT). Gene expression assay was … atahoranWebAug 26, 2024 · A gene expression assay of the positive ZmMRP-1 transgenic maize at different periods suggested that ZmMRP-1 was overexpressed only in the endosperm ... asian pfp memeWebApr 20, 2016 · Ectopic expression of mTOR mitigates miR-3188 suppression of NPC proliferation with inverse expression of cell-cycle pathway genes. MTT assays ( a ), EdU incorporation assays ( b ) and FACS assays ( c ) of NPC cells were performed after transfection with NC, ectopic mTOR or miR-3188 as indicated. atahoe