WebOct 4, 2024 · The FLAG-tagged protein identifies and binds to the FLAG tag-specific monoclonal antibody conjugated on a magnetic bead or agarose gel. Then, residual impurities are washed away, and the FLAG-tagged proteins are eluted using an affinity column with a low pH buffer or a high concentration of FLAG tag peptide. WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

Flag Tag Monoclonal Antibody - Cusabio

WebAnti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse Monoclonal Images Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38 (Figure F). WebAntibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope. ELISA. Use a concentration of 1 - 5 µg/ml. As coating antibody in sELISA. software for autistic child

DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™

http://www.abpbiosciences.com/product/flag-tag/ http://www.engibody.com/products/DYKDDDDK-FLAG-tag-Mouse-mAb-conjugated-Agarose-Beads-AT0078.html WebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ... software for automobile design