Web产品描述 Hieff Trans ® 通用型转染试剂 是基于最新的纳米技术研发的一款多用途、便捷高效的脂质体转染试剂,适用于 DNA 、 RNA 和寡核苷酸的转染,对难转染细胞 也 具有较高的转染效率。 其独特的配方使其可直接加入培养基中,血清的存在不会影响转染效率,这样可以减少去除血清对细胞的损伤。 Web12 de abr. de 2024 · DMEM/F-12 with no phenol red was purchased from HyClone Proteins (Logan, UT, USA). Hieff Trans Liposomal Transfection Reagent and DH5α chemically competent cells were purchased from Yeasen Biotechnology (Shanghai, China).
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Web8 de mar. de 2024 · Lentiviruses were produced by co-transfection of the expression vector of interest with the packaging plasmids psPAX2 and pMD2G (Addgene) into HEK-293 cells using Hieff Trans™ Liposomal Transfection Reagent (Cat#40802, Yeasen, China). Virus supernatant was collected after 48 h. Transfected MM cells were screened by puromycin. WebHieff Trans®通用型转染试剂是基于最新的纳米技术研发的一款多用途、便捷高效的脂质体转染试剂,适用于DNA、RNA和寡核苷酸的转染,对难转染细胞也具有较高的转染效率。 … raw materials of anaerobic respiration
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Web30 de set. de 2024 · The siRNAs (50–100 n m) were introduced into cells using Hieff Trans liposomal transfection reagent following the manufacturer’s protocol (Yeasen). Cells were harvested 48–72 h after transfection... Web国产GMP级转染试剂助力细胞基因治疗的持续快速发展细胞基因治疗(Cell and Gene Therapy, CGT)作为生命科学研究最前沿的应用技术领域,分为细胞治疗和基因治疗两大部分。与常规的药物_国产GMP级转染试剂助力细胞基因治疗的持续快速发展_来宝网 Web20 de mai. de 2024 · Hieff Trans™siRNA/miRNA体外转染试剂是一种非脂质体PEI阳离子、两性分子转染试剂,为siRNA转染到哺乳动物细胞中而开发。 适用于siRNA和miRNA的 … simple house layout designer free