Irf3 phosphorylation antibody

Author: mbeu

August undefined, 2024

WebKnockout Tested Rabbit recombinant monoclonal IRF3 antibody [EPR2418Y]. Validated in WB, IHC, ICC/IF, Flow Cyt (Intra) and tested in Mouse, Human. ... DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization ... WebAntibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Peptide array. These antibodies target IRF3 in Human, Mouse, Rat, Non-human primate and Porcine samples. Our IRF3 monoclonal, polyclonal, recombinant monoclonal and recombinant ...

RNF39 mediates K48-linked ubiquitination of DDX3X and inhibits …

WebMar 21, 2024 · IRF3 (Interferon Regulatory Factor 3) is a Protein Coding gene. Diseases associated with IRF3 include Encephalopathy, Acute, Infection-Induced 7 and Encephalopathy, Acute, Infection-Induced 10 . Among its related pathways are Cytosolic sensors of pathogen-associated DNA and MyD88 dependent cascade initiated on … WebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IRF3 protein in your experimental model system, you can verify pathway activation in your cell lysates. iphone 11 timi

Phospho-IRF3 (Ser14) Antibody Affinity Biosciences

WebApr 11, 2024 · Since EhaF-mediated inhibition of TLR4-TRIF-IFN pathway occurs at the level of IRF3 phosphorylation ... Cells were lysed after 1.5 h and IRF3 was immunoprecipitated with anti-IRF3 antibody (1:100 ... WebMar 4, 2024 · IRF3 is a transcriptional factor that mediates expression of a large array of genes after viral infection. We hypothesized that DUBs might regulate the activity or stability of IRF3 and thereby facilitate or inhibit cellular antiviral responses. Web该IRF3 Polyclonal Antibody. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha … iphone 11 things to do

Recombinant Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) - Abcam

WebIRF3 and IRF7 Phosphorylation in Virus-infected Cells Does Not Require Double-stranded RNA-dependent Protein Kinase R or IκB Kinase but Is Blocked by Vaccinia Virus E3L Protein* - Journal of Biological Chemistry MECHANISMS OF SIGNAL TRANSDUCTION Volume 276, ISSUE 12, P8951-8957, March 2001 WebWestern blot analysis of Phospho-IRF3 pSer385 using Phospho-IRF3 pSer385 polyclonal antibody (Product # PA5-36775) at a dilution of 1:500. Lane 1: Hela cell lysate treated with PMA (100nM, 15min), Lane 2: … iphone 11 thickness mmWebNational Center for Biotechnology Information iphone 11 tim plano

"WebNov 29, 2024 · This phosphorylation event causes IRF3 to dimerize, translocate into the nucleus, and induce the expression of IFN-β. IFN-β then induces the expression of IFN-stimulated genes (ISGs) through... " - Irf3 phosphorylation antibody

Irf3 phosphorylation antibody

Inhibition of IRF3 phosphorylation and nuclear translocation in …

WebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI … WebInvitrogen Anti-Phospho-IRF3 (Ser396) Polyclonal, Catalog # 720012. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts with Human …

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WebMar 29, 2024 · IRF3 is activated by phosphorylation (by kinases TBK1 and IKKε), promoting dimerization, nuclear translocation, ... Anifrolumab, a blocking antibody against the IFN receptor (as opposed to targeting IFN-α isoforms), has reportedly better efficacy, although responses are far from complete . Another contribution IFN-driven disease that cannot ... WebMar 23, 2001 · We have investigated the mechanisms of phosphorylation of IRF7 and its close relative, IRF3, during activation of IFN gene expression following Newcastle disease virus (NDV) infection. By all parameters tested, IRF3 and IRF7 appeared to be activated by the same mechanism.

WebApr 14, 2003 · Viral infection results in the phosphorylation of IRF3, leading to its dimerization and interaction with the coactivators CREB-binding protein (CBP) and p300 … Web), the data reported here suggest that additional signals stimulate phosphorylation of IRF3 and IRF7, essential events in induction of the protective IFN response. This evidence is …

WebMar 12, 2015 · IRF3 activation requires phosphorylation, dimerization and nuclear translocation. However, the mechanisms for the termination of IRF3 activation in nucleus are elusive. Here we report the identification of TRIM26 to negatively regulate IFN-β production and antiviral response by targeting nuclear IRF3. WebMar 5, 2024 · Rnf39 deficiency and knockdown both enhanced RNA virus–induced IRF3 phosphorylation (Fig. 2, A to C, and fig. S3D). Furthermore, ... The cell lysates were immunoprecipitated with Flag antibody and then immunoblotted with the indicated antibodies. The specific bands were marked by asterisk. All experiments were repeated at …

WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) View more associated products Overview Product name Anti-IRF3 (phospho S386) antibody [EPR2346] See all IRF3 primary antibodies Description Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) Host …

WebViral infection results in phosphorylation of IRF3, leading to its translocation to the nucleus, where it activates promoters containing IRF3-binding … iphone 11 timsWebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation … iphone 11 timeout settingWebTBK1-dependent phosphorylation of IRF3 leads to its nuclear translocation and subsequent activation of transcriptional programs that ultimately lead to ... for 2 h at RT or over-night at 4°C. Primary antibodies used include: anti-pDNA-PKcs Ser2056 (ab124918, Abcam), anti-pDNA-PKcs Ser2056 (ab18192, Abcam) for mouse cell lines, anti-DNA-PKcs ... iphone 11 times out on hot spotWebKey features and details Rabbit polyclonal to IRF3 Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Western blot … iphone 11 timhWebThe induction of phosphorylation of STING on S366 of IRF3 on S396 was inhibited in T404E compared to WT or ... T404A, or T404E STAT2 were treated with cisplatin for 24 h. The cells were fixed then stained with IRF3 antibody (red) and DAPI for confocal analysis. (C) STAT2-deficient Calu-1 cells restored with WT, T404A, T404E, or Y690F STAT2 ... iphone 11 timWebMAVS then activates the kinase TANK-binding kinase 1 (TBK1), which phosphorylates IRF3 and IRF7, causing these transcription factors to homodimerize and enter the nucleus to turn on type I IFNs. MAVS also activates the kinase IKKβ, which activates nuclear factor kappa B (NF-κB) to induce proinflammatory cytokines. iphone 11 time to lockWebAnti-IRF3 antibody [IRF35I218] (ab50772) Datasheet Reviews ( 3) Q&A (4) References (9) Key features and details Mouse monoclonal [IRF35I218] to IRF3 Suitable for: ICC/IF, WB, Flow Cyt Reacts with: Human Isotype: IgG1 You may also be interested in Primary Alexa Fluor® 488 Anti-PSMA antibody [GCP05] (ab187570) iphone 11 times out too fast