Mcherry-gfp-lc3b
WebmRFP-GFP-LC3 腺病毒用于实时监测自噬 (流) mRFP 用于标记及追踪 LC3 ， GFP 的减弱可指示溶酶体与自噬小体的融合形成自噬溶酶体，即由于 GFP 荧光蛋白对酸性敏感，当自噬体与溶酶体融合后 GFP 荧光发生淬灭，此时只能检测到红色荧光。 这种串联的荧光蛋白表达载体系统直观清晰的指示了细胞自噬流的水平，是我们自噬研究尤其是自噬流研究不可或 … WebBeyotime adenovirus expressing mcherry gfp lc3b fusion protein recognizing cd46 Adenovirus Expressing Mcherry Gfp Lc3b Fusion Protein Recognizing Cd46, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Mcherry-gfp-lc3b
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WebHepG2 cells were transduced with mCherry-GFP-LC3 lentiviral vectors, which were pre-packed with the lentivirus packaging kit. The quenching properties of the GFP fluorescent signal in acid conditions (pH < 5) and the cherry fluorescent signal do not differ significantly in different acidic environments [ 28 ] when used to detect autophagic flux. Web摘要: 目的探讨顺铂对HeLa人宫颈癌细胞中Nrf2蛋白表达及核转位的影响，并观察细胞自噬和凋亡的变化。 方法使用不同浓度的顺铂(0、2.5、5、10 μmol/L)干预HeLa人宫颈癌细胞，MTT法检测细胞活性，Transwell实验观察细胞迁移能力，mCherry-GFP-LC3B融合蛋白腺病毒转染HeLa细胞观察自噬水平的变化，流式细胞术 ...
Web15 sep. 2024 · Main methods: The effects of M 4 IDP on proliferation, cell cycle and ROS production were determined by CCK-8 and flow cytometry assays. Annexin-V-FITC/PI, Hoechst 33258, MDC staining assays and Ad-mCherry-GFP-LC3B fluorescence assay were performed to investigate apoptosis and autophagy. Web21 jan. 2024 · HepG2 cells were transfected with a adenovirus expressing mCherry-GFP-LC3B fusion protein (Ad-mCherry-GFP-LC3B) (C3011, Beyotime Bio, China) according to the manufacturer’s instructions. After adenovirus transfection at a multiplicity of infection (MOI) of 20 for 24 h, the adenovirus-containing culture medium was removed and then …
WebDescription. pCMV-mCherry-GFP-LC3B is a plasmid developed by Biyuntian to express the red mCherry and green GFP double-labeled human LC3B fusion protein in mammalian cells. This plasmid contains the CMV promoter and is kanamycin resistant. After transfection, it can efficiently express the fusion protein of LC3B with the double label of … Web7 nov. 2015 · Plasmids construction pQCXIP-tfLC3B expressing mCherry-EGFP tandem fluorescent- tagged LC3B follows.First, mCherry gene amplifiedfrom pmCherry-C1 (Clontech, CA, USA) tem-plate PCR(up primer: -GTCGCGGCCGCCACCATGGTGAGCAA-GGGCGAGGAG-3 downprimer: -GCTGGATCCACCTCCGCTTCCA …
Web19 feb. 2024 · Flow cytometry software like Flow Jo can run a ratiometric calculation as a derived parameter, where autophagic flux is the mCherry signal divided by the GFP signal. This assay is simple and quick with a 1 …
Web14 apr. 2024 · Stable MCF10A cell lines carrying pBABE-puro-mCherry-EGFP-LC3B were established as described previously in the section of generation of stable cell lines. Cells were imaged using a Zeiss LSM 780 microscope with a 63X oil immersion objective lens, as described previously in the confocal section. mCherry and EGFP signals were analyzed … explanation of labelling mental healthWeb14 okt. 2024 · Ad-mCherry-GFP-LC3B was used to demonstrate blockage of the autophagic flux in eukaryocytes . Infection of Ad-mCherry-GFP-LC3B resulted in overexpression of tandem mRFP/mCherry-GFP proteins. In cells lacking an autophagic state, mCherry-GFP-LC3B was present in the cytoplasm as diffuse yellow fluorescence … explanation of laboratory testsWebFUW mCherry-GFP-LC3 (Plasmid #110060) Print Enlarge View all sequences Purpose To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes … bubble backsplash tileWebPlasmid EGFP-LC3 from Dr. Karla Kirkegaard's lab contains the insert LC3B and is published in PLoS Biol. 2005 May . 3(5):e156. This plasmid is available through … explanation of lab results for patientsWebQuantification of eGFP-mCherry-LC3B puncta G (n = 3). eGFP fluorescence was measured by flow cytometry in L6 cells stably expressing tandem fluorescent eGFP-mCherry-LC3B treated with with 250 μM FeCl 3 for 1, 2, 4 or 24 h after saponin permeabilization and cytoplasmic washout of the cells. eGFP dMFIs were quantified by subtracting the MFI of … explanation of knitting blanket sizesWebDescription. pCMV-mCherry-GFP-LC3B is a plasmid developed by Biyuntian to express the red mCherry and green GFP double-labeled human LC3B fusion protein in mammalian … bubble backpack catWeb3 apr. 2024 · Virus particles were collected after 48 h infection, filtered through a 0.22 μm membrane, added to HCT116 cells, and then incubated for 24 h at 37 °C. The medium was replaced and HCT116 cells were selected for puromycin tolerance (5 μg/mL) to obtain Caspase 3 knock-down, GFP-LC3B, or mcherry-EGFP-LC3B overexpression cells. explanation of lab results icd 10